The working principle of rotovap is pressure distillation, that is, under normal pressure or reduced pressure, heating at a constant temperature to make the rotating bottle rotate at a constant speed, and the substance is formed on the wall of the bottle to form a large-area film, which evaporates efficiently. The solvent vapor is cooled by a glass condenser and recovered in a collection bottle. According to the volume of the evaporating flask, rotovap can be divided into small (≤ 1, 2, 3, 5 L), medium (10, 20 L) and large (50 L).
The composition of rotovap:
The structure of rotovap is mainly composed of driving device, evaporating flask, collecting flask, condenser, vacuum device, lifting system, heating bath, control system, etc.
Extraction of cbd and bho in cannabis:
Mechoulam et al. found that CBD/BHO, CBD/BHO in industrial hemp mainly exists in the form of cannabidiol acid, and cannabidiol acid can be decarboxylated under conditions such as light, heating, alkalization, etc. and transformed into cannabis with pharmacological activity Diphenols. Organic solvents such as methanol, ethanol, acetone, and hexane are commonly used as the extraction solvent for cannabidiol. The extraction methods for cannabidiol include dynamic dipping, ultrasonic-assisted extraction, supercritical CO2 extraction (SPE), and microwave-assisted extraction. , Enzyme-assisted solvent extraction method, etc. CAI et al. used response surface methodology to optimize the concentration of eutectic solvent, solid-liquid ratio, extraction temperature, and extraction time to extract and separate cannabidiol from industrial hemp, and the extraction rate was as high as 12.22 mg/g. At present, the hot reflux extraction method, supercritical CO2 extraction method, dynamic impregnation method, and ultrasonic assisted extraction method are commonly used.
